Lecture 1, December 2, h 14.30-16.30,
1- What are Nanobodies? This part will explain how Nanobodies were discovered and give an overview of the unique properties of Nanobodies compared to those of conventional antibody fragments such as Fab, and ScFv.
2- How Nanobodies are generated and characterized? This lecture will introduce the different steps involved to generate and select Nanobodies against a given antigen, and to characterize them in term of affinity, specificity, epitope mapping, stability using various a range of technics including ELISA, SPR, Biolayer interferometry, ITC, fluorescence and circular dichroism
Lecture 2, December 4h 14.30-16.30,
How to engineer Nbs to further adapt their properties to the applications: creation of multivalent/multispecific Nanobodies, creation of chimeric Nbs (fusion with toxins, enzymes, etc.). We will discuss protein engineering strategies to improve the pharmacokinetics of Nbs and to label them for in vivo imaging.
Therapeutic, diagnostic and biotechnological application of Nanobodies, part 1
Lecture 3, December 6, h 14.30-16.30,
Therapeutic, diagnostic and biotechnological applications of Nanobodies, part 2
- Docente: Patrizia Polverino De Laureto